2024 Atga2ox8基因过表达载体构建及遗传转化植株表型分析

Atga2ox8基因过表达载体构建及遗传转化植株表型分析

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August undefined, 2024

WebDec 31, 2002 · Degradation of active C(19)-gibberellins (GAs) by dioxygenases through 2beta-hydroxylation yields inactive GA products. We identified two genes in Arabidopsis (AtGA2ox7 and AtGA2ox8), using an activation-tagging mutant screen, that encode 2beta-hydroxylases. GA levels in both activation-tagged lines were reduced significantly, and … WebNov 26, 2010 · The AtGA2ox8 (Genbank accession number: AL021960; Tair Web site accession number: At4g21200) gene from Arabidopsis thaliana cDNA was amplified using a reverse-transcription polymerase chain reaction (RT–PCR) method and specific 5′ (5′-TCC CCCGGG ATGGATCCACCATTCAACGAAATA T -3′) and 3′ (5′-CCG CTCGAG …

Overexpression of the gibberellin 2-oxidase gene from

WebWe found that CsGA2ox8, a gene whose product catalyzes the deactivation of bioactive GAs, was signiﬁcantly upregulated in treatment X1 compared to treatment X2. This expression difference was conﬁrmed by quantitative reverse … WebNov 14, 2014 · The tblastn program was run to identify the homologous gene sequences in all available switchgrass expressed sequence tag (EST) databases using the amino acid sequences of AtGA2ox8 , OsGA2ox5 (LOC_Os07g01340.1), OsGA2ox6 (LOC_Os04g44150.1) and OsGA2ox9 (LOC_Os02g41954.1). Phylogenetic trees and … immo livit herent

植物基因过表达的原理和载体构建 - 知乎 - 知乎专栏

WebNov 12, 2024 · The GA2ox gene, which belongs to a small multigene family, encodes GA 2-oxidase, the major catabolic enzyme in plants that converts the active GA to an inactive … WebAtGA2ox7 and AtGA2ox8 are more similar to each other than to other proteins encoded in the Arabidopsis genome, indicating that they may constitute a separate class of GA … WebJul 3, 2010 · Abstract Gibberellin 2-oxidase (GA 2-oxidase) plays very important roles in plant growth and development. In this study, the AtGA2ox8 gene, derived from … immo location 17 le bon coin

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Proper gibberellin localization in vascular tissue is required to ...

WebApr 28, 2024 · Abstract. Gibberellin (GA) hormones regulate the development of plants and their responses to environmental signals. The final part of GA biosynthesis is catalyzed by multifunctional 2-oxoglutarate-dependent dioxygenases, which are encoded by multigene families. According to their enzymatic properties and physiological functions, GA … WebApr 29, 2010 · Gibberellin 2-oxidase (GA 2-oxidase) plays very important roles in plant growth and development. In this study, the AtGA2ox8 gene, derived from Arabidopsis (Arabidopsis thaliana), was transformed and over-expressed in rapeseed (Brassica napus L.) to assess the role of AtGA2ox8 in biomass accumulation and lignification in plants. … immo lismont wellenWebFind the most current and reliable 7 day weather forecasts, storm alerts, reports and information for [city] with The Weather Network. list of trendy words

"WebMar 20, 2014 · Gibberellin (GA), a plant hormone, is involved in many aspects of plant growth and development both in vegetative and reproductive phases. GA2-oxidase plays a key role in the GA catabolic pathway to reduce bioactive GAs. We produced transgenic Arabidopsis plants expressing GA2-oxidase 4 (AtGA2ox4) under the control of a … " - Atga2ox8基因过表达载体构建及遗传转化植株表型分析

Atga2ox8基因过表达载体构建及遗传转化植株表型分析

Overexpression of BnGA2ox2, a rapeseed gibberellin 2-oxidase …

WebJul 13, 2024 · Both genes encode enzymes that have functional similarities to AtGA2ox7 and AtGA2ox8, which are class III GA 2-oxidases that 2β-hydroxylate C 20 -GAs. Previously unknown for GA 2-oxidases, AtGA2ox9 performs 2α-hydroxylation of C 19 -GAs and harbors putative desaturating activity of C 20 -GAs. Both genes encode enzymes that have functional similarities to AtGA2ox7 and AtGA2ox8, which are class III GA 2-oxidases that 2β-hydroxylate C20-GAs. Previously unknown for GA 2-oxidases, AtGA2ox9 performs 2α-hydroxylation of C19-GAs and harbors putative desaturating activity of C20-GAs.

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WebFeb 15, 2011 · Six C20 GA2ox genes, except for AtGA2ox7 and AtGA2ox8, were detected. Recent research demonstrated that improvements in plant architecture, such as semi-dwarfism, increased root systems and higher tiller numbers could be induced by overexpression of wild-type or modified C20 GA2ox in rice (Lo et al., 2008). C20 GA2ox … WebOverexpression of both AtGA2ox7 and AtGA2ox8 caused dwarfing ( Figures 1C and 1D), indicating that the substrates for these enzymes are required for normal elongation …

WebOct 30, 2024 · Key message Using a fixed RIL population derived from a widely used foxtail millet backbone breeding line and an elite cultivar, we constructed a high-density bin map and identified six novel multi-environment effect QTLs and seven candidate genes for dwarf phenotype. Abstract Plant height is an important trait that determines tradeoffs between … WebJan 1, 2024 · The homolog of CsGA2ox8 in Arabidopsis is AtGA2ox8, whose product functions in hydroxylating the C 20-GA precursors GA 12 and GA 53 15. In this study, consistent with the results from overexpression of AtGA2ox8 15, GA 12 and GA 53 levels dramatically decreased in OE8.1, suggesting that CsGA2ox8 has a function similar to …

WebApr 11, 2024 · Jo-Anne Theresa Glassock. July 4, 1946 - March 20, 2024 Dearly beloved Jo-Anne Theresa Glassock passed away on March 20, at the age of 76, in Laguna … WebWe identified two genes in Arabidopsis (AtGA2ox7 and AtGA2ox8), using an activation-tagging mutant screen, that encode 2beta-hydroxylases. GA levels in both activation-tagged lines were reduced...

WebJan 27, 2024 · 研究人员对小麦ABS3和ATG8同源蛋白的研究发现，ATG8-ABS3互作调控衰老的范式在双子叶和单子叶植物中都存在。. 基于ABS3亚家族基因促进衰老的途径与自 …

WebApr 30, 2024 · 为研究自噬相关基因 ATG8g 在香蕉中的潜在功能，以及在过表达植株中对植株形态发育的影响，本研究通过农杆菌转化法获得 MaATG8g 拟南芥过表达植株。结果 … list of triangle proofsWebJan 1, 2024 · We found that CsGA2ox8, a gene whose product catalyzes the deactivation of bioactive GAs, was significantly upregulated in treatment X1 compared to treatment X2. This expression difference was... list of tribal nations by stateWebApr 13, 2024 · 30DayWeather Long Range Weather Forecasts predict ideal conditions for a storm.; A Risky Day is not a direct prediction of precipitation (Rain/Snow) but instead a … list of triads musicWebAug 1, 2024 · GA2-oxidases (GA2oxs) are a class of 2-oxoglutarate-dependent dioxygenases that regulate the deactivation of bioactive GAs. In this study, we investigated the phylogeny and domain structures of the seven GA2ox genes present in the Arabidopsis thaliana genome. immo loches 37600WebJun 14, 2024 · We compared the expression changes of AtGA2ox7 or AtGA2ox8 between wild-type (WT) and zma-unmiR4-overexpressing (zma-unmiR4 OE) plants (Figure 3b). As shown in Figure 3c, AtGA2ox7 was significantly down-regulated while AtGA2ox8 displayed no obvious changes in both zma-unmiR4 overexpressors, suggesting that AtGA2ox7 … immolivit herentWebJan 1, 2003 · Abstract. Degradation of active C 19-gibberellins (GAs) by dioxygenases through 2β-hydroxylation yields inactive GA products.We identified two genes in … list of triangular numbers 1-100WebAug 3, 2010 · 2012-03-26 基因的克隆,转导,表达,在对目的基因pcr时,为什么一些人设... 8 2011-08-24 你好，请问我是要提取一段目的基因，后续要表达蛋白，在引物设计... 1 2012 … list of tribal revolt