Cpe binding site
WebNov 6, 2024 · When claudin-4 binder binds to the site of claudin-4 and C-CPE interaction, FRET signal is not detected. (b) Validation of the TR-FRET-based claudin-4 and C-CPE binding assay. The assay was ... WebSep 15, 2011 · Clostridium perfringens enterotoxin (CPE) causes the symptoms associated with several common gastrointestinal diseases. CPE is a 35 kDa polypeptide consisting of three structured domains, that is, C-terminal domain I (responsible for receptor binding), domain II (responsible for oligomerization and membrane insertion), and domain III …
Cpe binding site
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WebThe mRNA poly-A tail may also play a role in the cap-dependent translation. The cytoplasmic polyadenylation element (CPE) is a nucleotide sequence within the 3′ UTR … WebThe CPE binding protein, CPEB is localized at synapses and selectively concentrated in postsynaptic densities (PSDs). It has been proposed that CPEB-mediated cytoplasmic polyadenylation facilitates mRNA translation at synapses in an activity-dependent manner Wu et al (1998). CPEB-controlled local translation has been suggested to influence ...
WebThe action of CPE starts with its binding to receptors, which include certain members of the claudin tight junction protein family; bound CPE then forms a series of complexes, one of which is a pore that causes the calcium influx responsible for host cell death. Recent studies have revealed that CPE binding to claudin receptors involves ... WebApr 1, 2015 · Clostridium perfringens enterotoxin (CPE) is a three-domain polypeptide, which binds to Claudin-3 and Claudin-4 with high affinity. Because these receptors are highly differentially expressed in many human tumors, claudin-3 and claudin-4 may provide an efficient molecular tool to specifically identify and target biologically aggressive human …
WebApr 23, 2004 · The mechanism by which specific mRNAs are temporally activated is unknown, although both cytoplasmic polyadenylation elements (CPE) within … Cytoplasmic polyadenylation element (CPE)- and CPE-binding protein (CPEB)-independent mechanisms regulate early class maternal mRNA translational activation in Xenopus … WebSep 20, 2016 · The residues in the claudin-4-binding site of C-CPE_WT are shown by stick models. Full size image Figure 6: The mutation of S313′ to alanine in C-CPE alleviates …
WebMar 16, 2016 · Site-directed mutagenesis showed that an Asn residue located in this pentapeptide is a critical yes/no determinant for CPE binding, with adjacent residues modulating the affinity of CPE binding (note that …
WebApr 17, 2024 · CPE-binding protein 1 (CPEB1) is an RNA-binding protein that binds to CPE sequences and regulates translation of its target transcripts by inducing cytoplasmic manipulation of their poly(A) ... rowinski funeral home passaic njWebBinding of PABPs to the poly(A) tail enhances mRNA stability and translation. A variety of RBP binding to e.g., the cytoplasmic polyadenylation element (CPE), AU-rich Elements (ARE) or other element represented as XX. These RPB interact with polyadenylases or with the CCR4/NOT deadenylating complex. row in spark scalaWebApr 13, 2024 · Targeted binding of CpE to specific claudins, encoded by its C-terminal domain (cCpE), loosens TJ barriers to trigger molecular leaks between cells. Cytotoxicity … row in snowflakeWebOrb-binding sites are enriched in typical A-containing CPE motifs (light green), whereas Orb2 shows more preference for G-containing versions (dark green). The CPE variations used are listed on ... stream using phone to pcrow in the bibleWebJul 20, 2024 · Cytoplasmic polyadenylation requires the CPE binding site and hexanucleotide AAUAAA poly(A) signal (Sheets et al., 1994). Furthermore, the spatial arrangement of CPEs is important for their function (Pique et al., 2008). Two CPEs in close proximity (less than 50 nucleotides apart) or a CPE within 100 nucleotides of the poly(A) … row interchangesWebThe most common CPE sequence is UUUUAU, though there are other variations. Binding of CPE binding protein to this region promotes the extension of the existing polyadenine … rowin tape echo