Prime script ii 1st strand cdna synthesis kit
WebThe ProtoScript ® II First Strand cDNA Synthesis Kit is optimized mix for higher specificity and yield of cDNA. Recombinant M-MuLV reverse transcriptase with reduced RNase H … WebApr 11, 2024 · The purified RNA was reverse transcribed into cDNA using the HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) (Vazyme, Nanjing, China). The quality of the cDNA was verified by amplification of the reference genes ACT2 and PP2A from Arabidopsis and Eucalyptus using PCR.
Prime script ii 1st strand cdna synthesis kit
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http://www.genetics-jo.com/Products.aspx?Lng=1&id=49 WebThe first strand cDNA synthesized with this kit can be used for variety of applications, including second strand synthesis, hybridization, PCR amplification, and real-time PCR. II. …
WebTaKaRa prime script ii 1st strand cdna synthesis kit Prime Script Ii 1st Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, … WebApr 12, 2024 · Total RNAs were isolated from the tissues using TRIzol Reagent (Thermo Fisher Scientific) and converted to first-strand cDNA using the PrimeScript II 1st strand cDNA Synthesis Kit (Takara Bio, Shiga, Japan) and random hexamers as primers, according to the manufacturer’s instructions.
WebThe PrimeScript First Strand cDNA Synthesis Kit contains all of the reagents necessary for synthesis of first-strand cDNA from total or poly(A)+ RNA using PrimeScript Reverse … http://www.utopbio.cn/archives/date/2024/04/13/page/2
Web1.2. First Strand cDNA Synthesis Reaction 1.2.1. Assemble the first strand synthesis reaction on ice by adding the following components to the fragmented and primed RNA from Step 1.1.4: COMPONENT VOLUME Fragmented and primed RNA (Step 1.1.4) 10 µl (brown) NEBNext Strand Specificity Reagent 8 µl (lilac) NEBNext First Strand Synthesis Enzyme ...
Web1st strand cDNAを合成するために必要な全ての試薬が含まれており、すぐに実験を始めることができる。 PrimeScript RTaseは、M-MLV (Moloney Murine Leukemia Virus) 由来 … side dishes for rack of lambWeb2.6.2. cDNA Synthesis and Concentration Measurement First-strand cDNA synthesis was performed using the Affinity Script Multiple Temperature cDNA Synthesis Kit (Agilent Technologies). cDNA was prepared using 1.2 μg of target … the pines school twitterWeb1.2. First Strand cDNA Synthesis Reaction 1.2.1. Assemble the first strand synthesis reaction on ice by adding the following components to the fragmented and primed RNA … the pines school berkshireWebApr 13, 2024 · Lumiprobe官网lumiprobe中国官网 Lumiprobe中国代理商lumiprobe荧光染料授权代理商 the pines school marsh hillWebThe PrimeScript 1st strand cDNA Synthesis Kit yields cDNA up to 12 kb in length from total or poly A+ RNA, and is also recommended for GC-rich RNA templates. We use cookies to … side dishes for roast lamb dinnerWebTaKaRa primescripttm ii 1 st strand cdna synthesis kit Primescripttm Ii 1 St Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, … the pines school birmingham addressWebMay 29, 2024 · First-strand cDNA was synthesized using PrimeScript ™ RT reagent Kit (TaKaRa, Beijing, China). The relative expression of genes was calculated using the 2 −ΔΔCT method. 2.6. PAL Gene Cloning and Bioinformatics Analysis. cDNA was synthesized with PrimeScript II first-strand cDNA synthesis kit ... the pines school formby