WebElectron microscopic analysis of such intermediates linearized with either Hpa I or BamHI indicates that RSF1030 replicates in vitro from a unique origin located 70% from one end … WebThe vector also carries the RSF1030-derived RSF replicon, lacI gene and kanamycin resistance gene (KnR). This vector can be used in combination with pACYCDuet™-1 , pCDFDuet™-1 , and pETDuet™-1 in an appropriate host strain for the coexpression of 4 …

Structural and functional organization of ColE2 and ColE3 …

WebRSF 1030 that includes the origin of DNA replication was determined using the DNA of a small derivative, pST19. The nucleotide sequence of the pST 19 origin region is very … WebOrigin and Initiation Sites of λdv DNA Replication In Vitro T. Tsurimoto, H. Kouhara, K. Matsubara Biology 1985 TLDR Regions that have long been suspected to participate in λ DNA replication initiation, ice and oop were not required for the O, P-dependent λ-specific replication initiation. misu the cat

Anatomy of the Replication Origin of Plasmid ColE2-P9 - Semantic …

WebDownload scientific diagram Vector map various vectors used for cloning and expression (a) pHIL D2, (b) pRSF-2, and (c) pET-46, showing various restriction sites (www.snapgene.com). from ... WebpLZ1 4712 bp 600 1200 1800 2400 3000 3600 4200 T7 promoter; not functional for T7 RNA polymerase null strain; IPTG inducible promoter lac operator and LacUV5; compatible with T7 RNA polymerase null strain LacZ rrnB T1 terminator rrnB_T2 terminator fd terminator S-Tag T7 terminator RSF origin; copy number above 100 per cell AmpR promoter RSF ori … WebSep 1, 2015 · RSF1030 origin plasmids are compatible with standard cloning plasmids. • The RSF1030 origin plasmids are available in both medium and high copy versions. • A series of arabinose inducible expression vectors with the differing RSF1030 origins have been constructed with a wide variety of antibiotic selections. • The plasmids are freely ... infotic